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Description Uses
SEPHADEX G-50
SEPHADEX G-50 structure
CAS No.
9048-71-9
Chemical Name:
SEPHADEX G-50
Synonyms
PDXTM G.F.;SEPHADEX G-50;SEPHADEX(R) G-50;SEPHADEX G-50 FINE;PDX GF 50 (50-150);PDX GF 50 (100-300);SEPHADEX G-50 COARSE;Sephadex G-50 60-100;SEPHADEX G-50 MEDIUM;Sephadex G-50 100-200
CBNumber:
CB3728066
Molecular Formula:
NULL
Formula Weight:
0
MOL File:
Mol file
SAFETY
  • Risk and Safety Statements
WGK Germany  3
HS Code  39140000

SEPHADEX G-50 Chemical Properties,Uses,Production

Description

Sephadex G-50 Superfine is a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The Superfine´s small bead size give higher efficiency.
  1. Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  2. Suitable for purification of DNA from small molecules by gel filtration
  3. Small bead size for shorter diffusion distances
  4. Smallest bead size in the Sephadex G-50 range
Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

Uses

The media used for gel exclusion chromatography include dextran (Sephadex™), polyacrylamide (Bio-Gel P™) and dextran-polyacrylamide (Sephacryl™) and agarose (Sepharose™ and BioGel A™). Each is available with a variety of different ranges of pore size in the beads, permitting separation of macromolecules of different size.
A gel with a smaller range of pore sizes (and hence a smaller range over which it can separate macromolecules on the basis of their size) will give a higher resolution; a gel with a wider range will give lower resolution, but will permit fractionation of a larger range of sizes as an initial step when the approximate molecular mass of the enzyme is not known.
The particle size of the gel beads (the mesh size) also affects resolution; smaller beads permit higher resolution, but a lower flow rate through the column (and hence a slower separation).
The table shows the useful range for the most commonly used gel filtration media - the lower and upper molecular sizes (in kDa) over which they can be used to separate macromolecules. The upper limit is known as the exclusion limit of the gel - the size above which proteins will elute in the void volume of the column.
 
lower limit
upper limit
dextran gels
Sephadex G-50
1.5
30
Sephadex G-75
3
80
Sephadex G-100
4
150
Sephadex G-150
5
300
Sephadex G-200
5
600
polyacrylamide gels
Bio-Gel P-10
1.5
20
Bio-Gel P-30
2.5
40
Bio-Gel P-60
3
60
Bio-Gel P-100
5
100
Bio-Gel P-150
15
150
BioGel P-200
30
200
Bio-Gel P-300
60
400
dextran-polyacrylamide gels
Sephacryl S-200
5
250
Sephacryl S-300
10
1500
Sephacryl S-400
20
8000
agarose gels
Sepharose 6B
10
4000
Sepharose 4B
60
20,000
Sepharose 2B
70
40,000
Bio-Gel A-0.5
10
500
Bio-Gel A-1.5
10
1500
Bio-Gel A-5
10
5000
Bio-Gel A-15
40
15,000
Bio-Gel A-50
100
50,000

SEPHADEX G-50 Preparation Products And Raw materials

Raw materials

Preparation Products


SEPHADEX G-50 Suppliers

Global( 48)Suppliers
Supplier Tel Fax Email Country ProdList Advantage
Shanghai Zheyan Biotech Co., Ltd.
18017610038
[email protected] CHINA 3623 58
career henan chemical co
+86-371-86658258
[email protected] CHINA 30004 58
BOC Sciences
1-631-619-7922
1-631-614-7828 [email protected] United States 20115 58
Chongqing Chemdad Co., Ltd
+86-13650506873
[email protected] CHINA 30864 58
future industrial shanghai co., ltd 400-0066-400;021-60496031
021-55660885 [email protected] China 2040 65
Nanjing Chemlin Chemical Co., Ltd 025-83697070
+86-25-83453306 [email protected] China 19977 64
BEST-REAGENT 400-1166-196 18981987031
028-84555506 QQ:800101999 [email protected] China 9900 57
Tianjin heowns Biochemical Technology Co., Ltd. 400 638 7771
[email protected] China 14460 57
Sinopharm Chemical Reagent Co,Ltd. 86-21-63210123
86-21-63290778 86-21-63218885 [email protected] China 9847 79
Hunan Hui Bai Shi Biotechnology Co., Ltd. 0731-85526065
0731-85526065 [email protected] China 2731 62

View Lastest Price from SEPHADEX G-50 manufacturers

Image Release date Product Price Min. Order Purity Supply Ability Manufacturer
2018-12-25 Sephadex G-50
9048-71-9
US $1.00 / kg 1kg 95%-99% 1000kg career henan chemical co

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